Journal: Synthetic and Systems Biotechnology

Article Title: AmXlnR, a transcription factor involved in xylan degradation and pentose catabolism, enhances pullulan production from xylose in Aureobasidium melanogenum

doi: 10.1016/j.synbio.2026.02.007

Figure Lengend Snippet: In vivo G FP expression analysis control of the native and mutated promoters of AmXLN2 , AmBXL2 and AmABF1 . ( a ) Schematic representation of the native promoters and their variants with deleted putative XlnR-binding sites: P XLN2 Δ:P XLN2 with 5′-GGCTGA-3′ deleted; P BXL2 Δ:P BXL2 with 5′-GGTTAA-3′ deleted; P ABF1 Δ:P ABF1 with 5′-GGCTAT-3′ deleted. ( b ) Relative fluorescence intensity, ( c ) relative GFP transcriptional level, and ( d ) bright-field and corresponding fluorescence images of the reporter strains. Three independent replicates were performed for the statistical analysis. ∗ P < 0.05,∗∗ P < 0.01, ns: no significance.

Article Snippet: GFP fluorescence intensity was visualized using an Olympus U-LH100HG fluorescent microscope and quantified using a BioTeK Synergy H1 Hybrid Reader (BioTek Instruments Inc., USA) (485 nm excitation and 520 nm emission).

Techniques: In Vivo, Expressing, Control, Binding Assay, Fluorescence

Journal: bioRxiv

Article Title: Extending the limits of 3D printed polymers on paper towards bioanalytical sensing

doi: 10.64898/2026.03.27.714910

Figure Lengend Snippet: A . Comparison of 1800µm PP 3D µPAD actual channel widths after thermal curing for 0.2, 0.25, 0.3 and 0.35mm nozzles (n=4 discrete devices) B . Fluorescence image samples for each nozzle size after deposition of ThT within the channels. **p<=0.01.

Article Snippet: After running the assay with n=3 discrete devices for each concentration, fluorescence images of the sensing regions were captured with a Nikon Eclipse Ti2 microscope and fluorescence intensity quantified using Image J Fiji.

Techniques: Comparison, Fluorescence

Journal: bioRxiv

Article Title: Extending the limits of 3D printed polymers on paper towards bioanalytical sensing

doi: 10.64898/2026.03.27.714910

Figure Lengend Snippet: A . Schematic showing implementation of the G4-dimer formation assay, the G4 dimer ssDNA is deposited on (1a), control oligo on (1b), each will wick to the sensing region with ThT (2a and 2b) and (3a and 3b are the waste collection pads B . fluorescence images of each sensing pad used to quantify fluorescence intensity for five concentrations from 20nM to 100nM C. Results of fluorescence intensity quantified using Image J. *p<=0.05, **p<=0.01, ***p<=0.001

Article Snippet: After running the assay with n=3 discrete devices for each concentration, fluorescence images of the sensing regions were captured with a Nikon Eclipse Ti2 microscope and fluorescence intensity quantified using Image J Fiji.

Techniques: Tube Formation Assay, Control, Fluorescence